Background. Raman microscopy is a label-free chemically-selective technique with superb spatial resolution. Even though technological developments in Raman microscopy are impressive in terms of speed [1], there is still the issue of overwhelmingly large data sets generated, an aspect that fundamentally precludes video-rate microspectroscopy. In recent years, we developed a set of new computational microscopy tools exploiting paradigm shifts in signal processing (Compressive Sensing) in order to achieve chemically selective imaging at high speeds. We coin this set of methods compressive Raman microspectroscopy [2]: we design an experiment which retrieves the same outcome as in traditional Raman imaging, however performing considerably fewer measurements. For that purpose, we develop novel spectrometers layout using fast digital micromirror devices (DMD). Exploiting this emerging technology, we have shown record acquisition speeds and sensitivity in various biomedical systems. Recent outcomes allowed us to reach video-rate speeds for imaging in spontaneous Raman imaging [3].

As a next step, we will further increase the acquisition speed to tackle motile biological systems. In particular, we aim at imaging biofilm formation in bacteria biofilms. The successful applicant will work on a new spectrometer layout, in order to increase the dynamic range for imaging species at low and high concentration levels with compressive Raman imaging. This would allow to observe the formation of bacterial biofilms [4] in real time.

Candidates profile. Various PhD/Post-doc positions are open. Regardless of the position applied, the candidate must be ambitious and highly motivated, have a particular taste for optics and interdisciplinary research. Regarding the PhD position, he/she should have a degree in Physics or closely related area. Regarding the Post-Doc position, he/she should have a PhD in Physics, Physical Chemistry, or closely related area (e.g. Signal Processing, Materials Science), be highly independent (both at the organizational and scientific level) and aspiring supervisor. Programming skills are compulsory. Candidates from Life Sciences are also encouraged to apply. Previous experience with Raman spectroscopy is a plus.

Offer details. The Post-Doc is for 2-year contract (with possibility of renewal). The PhD position is for 3 years. Both positions include all social benefits in France (health care, pension system, unemployment aid etc.). Post-doc gross salary will vary depending on the experience. The position is to start as soon as possible, but will stay open until suitable candidates are found.

How to apply. These positions are part of the activities of the Complex Media Optics Lab, at the Laboratoire Kastler-Brossel, ENS, Paris. Applicants interested within these research activities should submit CV, a brief motivation statement, and at least one contact detail for requesting a recommendation letter, to the point of contact: Hilton Barbosa de Aguiar, h.aguiar@lkb.ens.fr .

References [1] J.-X. Cheng and X. S. Xie, Science 350(6264), (2015) [2] P. Berto et al, Opt. Lett. 42(9), 1696 (2017); C. Scotté et al, Anal. Chem. 90(12), 7197–7203 (2018); F. Soldevila et al, Optica 6(3), 341 (2019); B. Sturm et al, ACS Photonics 6(6), 1409–1415 (2019) [3] Gentner et al, arXiv:2301.07709 [4] P. Thomen et al, Soft Matter 16(2), 494–504 (2020); P. Thomen et al, PLoS One 12(4), e0175197 (2017)

Job Posting Title: Core Facilities Technician, Center for Biomedical Research Support

Hiring Department: Center for Biomedical Research Support

Position Open To: All Applicants

Weekly Scheduled Hours: 40

FLSA Status: Exempt

Earliest Start Date: Immediately

Position Duration: Expected to Continue

Location: AUSTIN, TX

Job Details: GENERAL NOTES: The Center for Biomedical Research Support (CBRS) is a collection of 10 service centers that operates under the Vice President for Research, Scholarship and Creative Endeavors (OVPR) and broadly supports the research community on campus and externally. CBRS serves a central role across all of campus to provide access to cutting-edge technology, expert advice, and centralized services. CBRS is comprised of core facilities including Biomedical Imaging, Bioinformatics, Biological Mass Spectrometry, Biomedical Research Computing, Cryo-EM imaging, Genome Sequencing, Microscopy/Flow Cytometry and Mouse Genetic Engineering. In addition, CBRS provides laboratory support in the forms of stockrooms, electronics repair, and external services drop boxes.  The Microscopy and Imaging Facility (MIF) is a core facility in the Center for Biomedical Research Support at the University of Texas at Austin.  Our mission is to support multidisciplinary research and further scientific knowledge by providing access to state-of-the-art light microscopy, flow cytometry, and other research instrumentation; consultations with our expert staff; instrument trainings; and educational seminars and workshops. We are seeking a skilled and motivated Core Technician to join our team. In this role, you will be responsible for the maintenance, operation, and user training of scientific instrumentation, with a specific focus on light microscopes, flow cytometers, sectioning instruments, plate readers, and gel imagers.  As a member of our team, you will play a critical role in advancing research projects at UT Austin. We provide excellent customer service to our researchers, and we believe in making our instrumentation accessible to any researcher, no matter their previous experience level or knowledge. By training researchers in this role, you will contribute to the development of the next generation of scientists.  Through working in a multidisciplinary core facility, you will gain experience with a wide variety of sample types and experiments. We are looking for someone who can work full-time on-site in the Microscopy and Imaging Facility to provide instrument support and maintenance when needed. UT Austin offers a competitive benefits package that includes:  

• 100% employer-paid basic medical coverage  
• Retirement contributions
• Paid vacation and sick time
• Paid holidays Please visit our Human Resources (HR) website to learn more about the total benefits offered. PURPOSE:  This position will support research at the University of Texas at Austin by providing user training on instrumentation, consultations with users on their experiments, and maintenance of instrumentation to ensure data quality, with a specific focus on light microscopes, flow cytometers, sectioning instruments, plate readers, and gel imagers. RESPONSIBILITIES: 
• Trains researchers on the theory and proper operation of instrumentation in the Microscopy and Imaging Facility, with a specific focus on light microscopes, sectioning instruments, gel imagers, and plate readers. Provides ongoing support to researchers for troubleshooting or experiment questions after training.
• Conducts daily and periodic maintenance of instruments, including light microscopes, flow cytometers, sectioning instruments, gel imagers, plate readers, and related computers. Schedules and coordinates external service technicians as needed. Keeps accurate records of all maintenance performed to ensure quality of experiment data.
• Operates instruments to acquire data on behalf of researchers. Communicates regularly with researchers on these assisted use projects to discuss their experiment needs and goals.
• Consults with researchers on experiment design, data acquisition, and analysis.
• Develops standard operating protocols as needed for new instrumentation or changes in methodology. Keeps accurate records of instrument usage and scheduling.  
• Other related functions as assigned. REQUIRED QUALIFICATIONS: 
• Bachelor’s degree with at least two years of relevant experience or master’s degree.
• Hands-on experience operating a cryostat or similar sectioning instrument.
• Hands-on experience operating a plate reader and/or gel imager.
• Hands-on experience operating light microscopes, including fluorescence microscopes.
• Ability to work independently to complete responsibilities.
• Organizational and time management skills.
• Excellent interpersonal skills.
• Excellent written and verbal communication skills. Relevant education and experience may be substituted as appropriate. PREFERRED QUALIFICATIONS:  
• Experience with light microscope maintenance protocols, including cleaning.
• Experience with flow cytometry maintenance protocols, including quality control.
• Experience training others to use scientific instruments.
• Experience with sample preparation, including fluorescent labeling. SALARY RANGE:  $45,000 + depending on qualifications WORKING CONDITIONS:
• Uniforms and/or personal protection equipment (furnished)
• May work around chemical fumes
• May work around standard office conditions
• May work around biohazards
• May work around chemicals
• Repetitive use of a keyboard at a workstation
• Use of manual dexterity
• Lifting and moving REQUIRED MATERIALS: Please mark "yes" on the application for required materials. Failure to attach all additional materials listed may affect candidates being considered for the position.
• Resume/CV
• 3 work references with their contact information; at least one reference should be from a supervisor.

• Letter of interest Important for applicants who are NOT current university employees or contingent workers: You will be prompted to submit your resume the first time you apply, then you will be provided an option to upload a new Resume for subsequent applications. Any additional Required Materials (letter of interest, references, etc.) will be uploaded in the Application Questions section; you will be able to multi-select additional files. Before submitting your online job application, ensure that ALL Required Materials have been uploaded.  Once your job application has been submitted, you cannot make changes. Important for Current university employees and contingent workers: As a current university employee or contingent worker, you MUST apply within Workday by searching for Find UT Jobs. If you are a current University employee, log-in to Workday, navigate to your Worker Profile, click the Career link in the left hand navigation menu and then update the sections in your Professional Profile before you apply. This information will be pulled in to your application. The application is one page and you will be prompted to upload your resume. In addition, you must respond to the application questions presented to upload any additional Required Materials (letter of interest, references, etc.) that were noted above.

  Employment Eligibility: Regular staff who have been employed in their current position for the last six continuous months are eligible for openings being recruited for through University-Wide or Open Recruiting, to include both promotional opportunities and lateral transfers. Staff who are promotion/transfer eligible may apply for positions without supervisor approval.

  Retirement Plan Eligibility: The retirement plan for this position is Teacher Retirement System of Texas (TRS), subject to the position being at least 20 hours per week and at least 135 days in length.

  Background Checks: A criminal history background check will be required for finalist(s) under consideration for this position.

  Equal Opportunity Employer: The University of Texas at Austin, as an equal opportunity/affirmative action employer, complies with all applicable federal and state laws regarding nondiscrimination and affirmative action. The University is committed to a policy of equal opportunity for all persons and does not discriminate on the basis of race, color, national origin, age, marital status, sex, sexual orientation, gender identity, gender expression, disability, religion, or veteran status in employment, educational programs and activities, and admissions.

  Pay Transparency: The University of Texas at Austin will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor’s legal duty to furnish information.

  Employment Eligibility Verification: If hired, you will be required to complete the federal Employment Eligibility Verification I-9 form.  You will be required to present acceptable and original documents to prove your identity and authorization to work in the United States.  Documents need to be presented no later than the third day of employment.  Failure to do so will result in loss of employment at the university.

  E-Verify: The University of Texas at Austin use E-Verify to check the work authorization of all new hires effective May 2015. The university’s company ID number for purposes of E-Verify is 854197. For more information about E-Verify, please see the following:

• E-Verify Poster (English) [PDF]
• E-Verify Poster (Spanish) [PDF]
• Right To Work Poster (English) [PDF]

• Right To Work Poster (Spanish) [PDF]

  Compliance: Employees may be required to report violations of law under Title IX and the Jeanne Clery Disclosure of Campus Security Policy and Crime Statistics Act (Clery Act). If this position is identified a Campus Security Authority (Clery Act), you will be notified and provided resources for reporting. Responsible employees under Title IX are defined and outlined in HOP-3031. The Clery Act requires all prospective employees be notified of the availability of the Annual Security and Fire Safety report. You may access the most recent report here or obtain a copy at University Compliance Services, 1616 Guadalupe Street, UTA 2.206, Austin, Texas 78701.

The Ries lab is developing super-resolution microscopy methods for structural cell biology. In this project we will develop a fast, simple, and robust 3D and multi-color MINFLUX microscope with highest performance as open source.

An excellent opportunity is available for a Research Officer / Senior Research Officer to join the Centre for Dynamic Imaging at Australia’s pre-eminent biomedical research institute.

About the position The Centre for Dynamic Imaging (CDI) in the Advanced Technology and Biology Division, provides researchers with access to off-the-shelf and custom-built microscopy solutions, and specialist expertise to generate detailed, quantitative, and real-time views of biological systems for immune disorders and cancer, as well as infectious and inflammatory disease research. We are a multidisciplinary team of scientists, with expertise in biology, engineering, and physics. A dedicated team of bioimage analysts also provides access to customised analysis pipelines as well as virtual computing infrastructure with an array of software for the visualisation, management, and analysis of data. We work collaboratively with research teams to provide innovative solutions in experimental design, sample preparation, data acquisition and data analysis, with an aim to gain new insights and accelerate discoveries. The CDI is equipped with several light sheet microscopes, including the Zeiss Lattice light sheet and Z1 light sheet microscopes, both single molecule and 3D SIM super-resolution microscopes, several multiphoton platforms, equipment for spatial transcriptomics and proteomics, laser capture microdissection and an array of high-end confocal microscopes, such as the Leica Stellaris confocal with white light laser and FLIM. We are looking for an enthusiastic and self-directed person who is highly specialized in advanced microscopy techniques to apply their expertise in 3D multiplexed imaging of tissues using a range of approaches, including confocal, light sheet and multiphoton microscopy. The Research Officer / Senior Research Officer will have an opportunity to work collaboratively with research teams at the Institute working on a wide range of biomedical projects and will be contributing to high impact research and translation. This position provides an excellent opportunity to be involved in world-class research projects, and for the successful applicant to build themselves a major international reputation.

Responsibilities include, but not limited to:

• In close collaboration with research teams and other members of the CDI, you will have a major role in conceptualising, designing, and conducting experiments using advanced microscopy (including experimental design, data collection, interpretation, and presentation)
• Apply state of the art methods for the preparation of tissues and the use of immunofluorescence staining for 3D multiplexed imaging of tissues from mouse or patient biopsy material, including tissue clearing, immunofluorescence staining and the preparation of tissue whole mounts.
• Providing training and supervision to students, post docs and peers in the use of confocal, light sheet and multiphoton microscopy and on the visualisation, processing and analysis of data and interpretation of results.
• Operating, troubleshooting, and maintaining confocal, light sheet and multiphoton microscopes, including Zeiss LSM 880, 980 and Leica Stellaris platforms, a Z1 light sheet microscope and Olympus multiphotons.
• Providing support to the head of the CDI for the supervision of staff and students and for coordinating the completion of projects in a timely manner.
• Working collaboratively with staff (WEHI Lab heads, post-docs, RAs, students), to introduce or develop new approaches in the CDI for facility users, which allow us to push the boundaries in 3D imaging.
• Contributing to the preparation and delivery of presentations and manuscripts for publication.
• Providing oversight for the maintenance and compliance for all matters related to health and safety, animal and human ethics and other regulatory requirements.
• Keeping abreast of the latest technological advances in the field by attending conferences or workshops both nationally and internationally.
• Contributing to the analysis and processing of image data using software packages like ImageJ/FIJI or IMARIS.
• Exquisite and timely record keeping and data entry into Electronic Lab Notebook and clear presentation of results.
• Strong desire to foster a positive workplace culture, which provides staff and students with a safe and productive place to work.

Selection Criteria Essential

• PhD in biology, chemistry, physics, or related field of medical research (with evidence of experience/background in light microscopy such as confocal).
• Hands on problem solver that is proficient in scientific technology.
• Evidence of experience in the preparation of tissue for 3D imaging, including tissue fixation, clearing and immunofluorescence labelling.
• Knowledge and direct experience with state-of-the-art instrumentation for confocal, light sheet and multiphoton microscopy.
• Ability to troubleshoot experimental protocols, interpret data and design new experiments.
• Experience in managing research collaborations and the supervision of staff or students.
• Strong track record in the application of advanced microscopy to biological samples and to developmental or disease biology research.
• High level attention to detail and strong organisational skills, as well as excellent verbal and written communication skills.

Desirable

• Proficiency in using ImageJ/FIJI, IMARIS and other software tools.
• Experience in light sheet microscopy and/ or tissue clearing.
• A good knowledge of or experience in the steps required to process confocal and light sheet imaging data.
• Previous experience working within a core facility environment, and with individuals who have varying degrees of expertise in microscopy and who work on a diverse range of samples and experimental models.

Terms of appointment This position is available initially for 2 years in a full-time capacity, with potential for this position to become ongoing thereafter. Salary is Academic Level A6-A8 for a Research Officer ($97,152–$104,281) or for more experienced candidates, Academic Level B1-B6 ($109,778-$130,353). Up to 17% superannuation and very attractive salary packaging options are available.

Pre-application enquiries are encouraged and can be directed to Professor Kelly Rogers – rogers@wehi.edu.au or Dr Niall Geoghegan geoghegan.n@wehi.edu.au

A Senior Research Officer position description is available here. A Research Officer position description is available here.

How to apply Please apply through the WEHI careers page by uploading your CV, cover letter and a document addressing the key selection criteria in pdf format.

Application Closing Date:  6 May 2024

The FIB-SEM Collaboration Core (F-SCC) at Yale School of Medicine is seeking a talented, skilled, and highly motivated researcher to assume the role of Senior Staff Scientist (87647BR) to lead and perform the FIB-SEM sample preparation and image acquisition operations. At the forefront of volume Electron Microscopy, the F-SCC utilizes the state of the art enhanced Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) technology for large volume imaging with nanometer isotropic resolution. Under supervision of the Director, the candidate will be responsible for pipeline processes of X-ray guided FIB-SEM sample preparation, enhanced FIB-SEM image acquisition, and image registration and alignment. The candidate is expected to be multi-tasking, perform independent experiments using the state-of-the-art laboratory techniques including enhanced Focused Ion Beam Scanning Electron Microscopy and X-ray micro-CT. The candidate will work closely with supervisor, group members, and collaborators.

Johnson & Johnson is recruiting for a Principal Scientist, Cryo-EM, located in Spring House, PA.

At Johnson & Johnson, we believe health is everything. Our strength in healthcare innovation empowers us to build a world where complex diseases are prevented, treated, and cured, where treatments are smarter and less invasive, and solutions are personal. Through our expertise in Innovative Medicine and MedTech, we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow, and profoundly impact health for humanity. Learn more at https://www.jnj.com/

The Discovery Technologies and Molecular Pharmacology group is a dynamic, diverse and critical part of the Discovery Sciences organization in Janssen R&D. We are committed to the discovery and characterization of high-quality chemical leads needed for the generation of small molecule clinical compounds in all five Janssen Therapeutic Areas. This mission requires deep scientific expertise across broad disciplines including chemistry, cellular and molecular pharmacology, enzymology, screening, protein science, and structural biology coupled with an ability to work collaboratively with internal and external partners. Building on a strong legacy of success, we are currently seeking an outstanding and motivated individual to join our team as a Principal Scientist, Cryo-EM.

Key Responsibilities: Be an active member of drug discovery teams, working closely with computational and medicinal chemists on structure-based drug design approaches for small/large molecule optimization. Be responsible for end-to-end high-resolution structure determination via Cryo-EM including sample and cryo-EM grid optimization, data collection, processing, and structure refinement, and communicating these results to the project teams. Collaborate closely with protein scientists, biophysicists, protein NMR, and X-ray crystallography scientists as part of an integrated structural biology effort. Actively seek innovative strategies to handle complex targets including grid preparation, data collection, processing, and analysis. Contribute to increasing the structural biology scientific expertise of the group by publishing high-impact research papers, attending and presenting at scientific conferences, internalizing impactful outside technologies and techniques, and establishing academic collaborations. Mentor lab scientists and contribute to the strategic planning of the Structural Biology group. QUALIFICATIONS Education:

Ph.D. or equivalent and postdoctoral experience in structural biology (Cryo-EM, X-ray crystallography, biophysics, protein engineering) or related field is required Experience and Skills: Required:

Minimum 3 years of industry experience is required Deep scientific expertise in structural biology, with strong experience in sample preparation using Vitrobot, data collection via EPU, and structure determination are required Strong record of external publications and presentations is required Outstanding written and oral communication skills are required Preferred: Experience in cryo-ET/FIB milling, and Micro-ED is preferred Experience in machine learning and docking programs (e.g., Schrödinger) is preferred Experience in structure-guided small/large molecule design utilizing Cryo-EM/X-ray is preferred Knowledge of protein production techniques and protein stability requirements is preferred Broad knowledge of biophysics (such as ITC, DSF, SPR, ASMS, HDX-MS), biochemistry, molecular pharmacology, and protein sciences is preferred Expertise in one or more of the following therapeutic areas: Oncology, Inflammation, Neuroscience, Cardiovascular, and Infectious Diseases is preferred Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.

For more information on how we support the whole health of our employees throughout their wellness, career and life journey, please visit www.careers.jnj.com

"We seek an expert in multi-photon microscopy to direct and contribute to the integration of intravital microscopy into the research programs of multiple laboratories in the School of Medicine and Public Health. The position offers an exciting opportunity for a highly motivated scientist to collaborate and contribute to groundbreaking research in neuroscience and other disciplines. The Scientist will be responsible for liaising with multiple research groups, and customizing the design and execution of experiments utilizing live fluorescence imaging. The scientist will also be a vital contributor to data analysis, publication of research findings, and grant proposal preparation."

The CRUK Cambridge Institute's (CRUK CI) scientific Core Facilities perform a fundamental role at the heart of our research community. They are a repository for deep knowledge and expertise in scientific technologies and techniques and work in partnership with the Institute's research groups in answering biological questions related to cancer. The Light Microscopy Core Facility provides expertise, training, and instrumentation across a wide range of research projects. We support 5000 hours of yearly microscope usage for our 200 researchers at the CRUK CI and the wider community. The supported technologies include Confocal, High Content, Light Sheet, STED Super-resolution and Widefield Microscopy as well as a wide range of image analysis. We are looking for a Microscopy Specialist to join our team to provide scientific and technical support and training in microscopy and image analysis to members of the research groups. The role holder will assist with the day to day running of the Microscopy Core Facility including maintaining the microscopy equipment. You will have the opportunity to apply your problem-solving skills to troubleshoot challenging technical issues, analyse image data and contribute towards a large range of research projects in the CRUK CI. The Microscopy Specialist reports directly to the reports directly to the Light Microscopy Facility Manager, Andreas Bruckbauer and will be part of a team of currently four facility staff supporting research assistants, research associates, clinical fellows, and PhD students from the research groups. The role holder would possess in-depth experience in confocal microscopy and image analysis at a level sufficient to provide training to inexperienced users. Expertise in other advanced microscopy techniques such as Super-Resolution microscopy or Light Sheet microscopy is desirable (required for the Senior Scientific Associate). Ideally you would have experience of working in a Microscopy Core Facility team and will possess excellent communication and teamwork skills. Click the 'Apply' button below to register an account with our recruitment system (if you have not already) and apply online. Please ensure that you upload a covering letter and a CV in the Upload section of the online application. The covering letter should outline how you match the criteria for the post and why you are applying for this role. If you upload any additional documents which have not been requested, we will not be able to consider these as part of your application. Please include details of your referees, including email address and phone number, one of which must be your most recent line manager. The closing date for applications is: 6th May 2024 The interview date for the role is: To be confirmed Please quote reference SW41069 on your application and in any correspondence about this vacancy. The University actively supports equality, diversity and inclusion and encourages applications from all sections of society. The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

The Ries lab is developing super-resolution microscopy methods for structural cell biology. In a WWTF-funded project we will develop a new deep learning-based approach to reconstruct a movie of a cellular process from tens of thousands of snapshots taken with high-throughput super-resolution microscopy and are looking for a Postdoctoral Researcher with a background in machine learning.

The Ries lab is developing super-resolution microscopy methods for structural cell biology. In this project we will develop a fast, simple, and robust 3D and multi-color MINFLUX microscope with highest performance as open source.

The international Euro-BioImaging Bio-Hub team hosted by EMBL in Heidelberg, Germany, is seeking a dynamic individual with a background in administration and finance, ideally with previous experience in supporting international travel administration. This pivotal role involves supporting the Bio-Hub team in administering the finances of their growing portfolio of EU-funded projects as well as in supporting the organisation of external activities such as webinars, smaller in person events or training courses.

The international Global BioImaging team, operating within the Euro-BioImaging Bio-Hub hosted by EMBL in Heidelberg, Germany, is seeking a dynamic individual with a scientific management and, ideally, imaging background. This pivotal role involves supporting and coordinating our network of Global BioImaging partners (www.globalbioimaging.org), contributing to strategic initiatives, supporting our communication activities and ensuring seamless execution of administrative functions linked to the GBI activities. If you're passionate about advancing bioimaging research on a global scale and thrive in a collaborative environment, we invite you to apply and be part of our impactful mission (www.globalbioimaging.org).

The Max Planck Institute for Biological Intelligence (MPI-BI) is located in the southwest of Munich, with its two locations in Martinsried and Seewiesen. It is one of more than 80 independent research institutes of the Max Planck Society. With around 500 employees from more than 50 nations, we investigate the fundamentals of "biological intelligence". We aim to understand how, in the course of evolution, animals developed abilities to cope with a constantly changing environment and to find ever new solutions to problems.

Within other service units, the Imaging Facility supports scientists in many aspects of modern microscopy. Our aim is to maintain our state-of-the-art instruments, train scientists on various light setups, support them in establishing their experimental pipelines and develop new techniques or microscopes to tackle new challenges in modern neuroscience. We are looking for a: Microscopy Specialist (m/f/d).

Your responsibilities

• First level maintenance and troubleshooting of a variety of instruments including widefield,  confocal, spinning disk, 2-photon and lightsheet microscopes.
• Theoretical and practical training of institute members.
• Selection, installation and upgrade of commercial systems.
• Development of new optical hardware and applications as well as solutions for image processing and analysis.

  Your profile

• Diploma or Master’s degree in biophysics, physics or biology with a strong focus on microscopy, PhD is an advantage.
• Excellent knowledge of principles and methods of microscopy (including fluorescence, confocal and 2-photon microscopy, properties of light sources and detectors).
• Good skills in handling optics, microscopy hardware and software.
• High degree of motivation and self-reliance combined with excellent organizational and team-work skills.
• A strong sense for diligence and accuracy.
• Fluent in English, German is an advantage.

  What we offer

• An interesting, multifaceted and rewarding job in a scientific, modern and international environment.
• Opportunities for regular professional exchange, individual training and further qualification.
• Remuneration according to TVöD Bund, depending on qualifications, activities and experience.
• Social benefits of the public service (company pension scheme (VBL), annual bonus, subsidized MVV job ticket, group accident insurance).
• Modern workplaces and active company health management with health and sports facilities.
• Work-life balance (cooperation with a family service, flexible working hours).
• A diverse working environment where equal opportunities and diversity are actively promoted.

The position is initially limited to a period of 2 years, with the possibility of an extension or a permanent contract depending on the suitable candidate. The Max Planck Society strives for gender equality and diversity. Furthermore, the Max Planck Society wants to increase the proportion of women in areas in which they are underrepresented. Women are therefore expressly encouraged to apply. The Max Planck Society has set itself the goal of employing more disabled people who are thus explicitely encouraged to apply. If you have any questions, please contact Claudio Polisseni via claudio.polisseni@bi.mpg.de.

Your application

Did we attract your interest? Then please apply until 22.04.2024 by submitting your complete application documents via our online application portal. For more information, please visit our homepage at www.bi.mpg.de.                               MAX PLANCK INSTITUTE FOR BIOLOGICAL INTELLIGENCE Am Klopferspitz 18 | 82152 Planegg, Martinsried Eberhard-Gwinner-Strasse | 82319 Starnberg - Seewiesen

About Us: ZEISS Microscopy is the world's only one-stop manufacturer of light, electron, X-ray and ion microscope systems and offers solutions for correlative microscopy. The portfolio comprises of products and services for life sciences, materials and industrial research, as well as education and clinical practice. With its unique portfolio, constant innovations and strong partnerships, ZEISS Microscopy enables leading researchers to find answers to our society’s most pressing challenges and drive scientific discovery forward. A dedicated and well-trained sales force and a responsive service team enable customers to use their ZEISS microscopes to their full potential. Carl Zeiss Research Microscopy Systems (RMS) is the North American operation of the 177-year-old global organization, responsible for Sales, Service, Applications and Marketing for the United States and Canada.  To learn more about ZEISS RMS, visit:  https://www.zeiss.com/microscopy/en/about-us.html Here's What You'll Do: Our goal is to introduce students (later undergrad, masters or PhDs) to career paths in industry related to the company’s sales organization including roles directly in sales as well those in technical support, instrument service, business development, marketing, product management, customer service and leadership. The program is designed to give interns a holistic view of career paths in the organization and work directly with colleagues with technical and advanced degrees. You also will have the opportunity to interact with leading scientists and get hands on experience with cutting edge imaging technologies. This is a remote role (i.e., relocation to a ZEISS facility is not required) with a high degree of travel including daily trips to customer labs to do field work with sales, service and application teams, extended time (1-2 weeks) at our ZEISS Microscopy Customer Center in Dublin CA and a final trip to our headquarters in White Plains NY. Candidate will have a designated mentor and supervisor for duration of internship. There will be a project you work to develop over the course of your internship with your mentor and the team members you work with. The final project presentation will be delivered to the ZEISS leadership team in White Plains at end of the internship and you may share this afterwards as your work product for the internship. In addition to the stipend, all travel expenses will be reimbursed.  Note: this is not a R&D, Engineering, Programming or production internship. Location/Region: This position can be in one of the following areas; Baltimore/Washington, New York, Boston, Chicago, Texas, Bay Area, San Francisco, or Los Angeles. Do you qualify:

• Science or Engineering background; upper-level BS student or MS/PhD candidates.
• MS Office and presentation skills.
• Willingness to learn and work hands-on in a laboratory imaging environment.
• Valid driver's license. Nice to Haves:
• Familiarity with high-end microscopy, including laser scanning microscopy, and imaging techniques beyond simple image capture and manipulation.
• Familiarity in biological applications and workflow solutions, including image analysis and bioinformatics. The hourly pay range for this position is $20.00 – $21.25. The pay offered for this role may be influenced by factors such as job location, scope of role, qualifications, education, experience, & complexity/specialization/scarcity of talent.

About Us: Carl Zeiss Microscopy, LLC supports 18 summer courses from May through August in 2024 at the Marine Biological Laboratory (MBL) in Woods Hole, MA consisting of students and faculty attending from 30+ countries around the world. The Zeiss Training Group Summer Interns at MBL are an integral part of our summer support structure. Once interns are oriented, their primary role is to support student and faculty image acquisition activities throughout the summer. This includes basic set up of widefield microscopes, an understanding of Kohler illumination, brightfield, phase contrast, DIC and fluorescence contrasting methods, troubleshooting basic hardware and software issues, as well as conveying an understanding of what constitutes a good scientific image and how to prepare for and carry out image analysis. 

Here's What You'll Do: This will be an opportunity to learn, operate and support more advanced systems as well, including laser scanning confocal microscopy, Airyscan and potentially other super-resolution techniques. MBL Interns must have good analytic and time management skills, and be detail oriented and conscientious. Additionally, be able to work closely with people from a diverse range of cultural and scientific backgrounds. Inventory and QC along with tracking of equipment is critical. An outgoing disposition is an advantage, with a willingness to proactively engage with faculty and students. Working in overlapping shifts will be essential, so enjoying a strong team environment but also being able to work independently, are both essential for a successful internship. Do you qualify?

• Student currently enrolled in a Bachelor’s, Master’s or Ph.D. degree in biology, physics, optics, biochemistry or related field.
• Skilled in microscopy, optics, and imaging with a focus or interest in biology.
• Proficiency in computing hardware and software including Microsoft Office Suite (MS Word, MS Excel, PowerPoint).
• Ability to work independently for periods of time.
• Excellent verbal & written communication skills.​
• Must be able to lift, using proper form and technique, up to 50 pounds.
• Must be able and willing to work between 40 to 50 hours per week, including shift work.

• Nice to Haves:

• Experience with imaging and image analysis software preferred. The hourly pay range for this position is $15.50 – $17.00. The pay offered for this role may be influenced by factors such as job location, scope of role, qualifications, education, experience, & complexity/specialization/scarcity of talent.

The Department of Structural Biology at St Jude Children’s Research Hospital is seeking to hire a Director of the Single-Molecule imaging Center (SMC) to lead a team, comprised of a principle scientific computing engineer, staff scientific computing engineer and scientific computing engineer, whose backgrounds span from computer science and mathematics to mechanical and electrical engineering. The ideal candidate has extensive experience developing and utilizing single-molecule fluorescence microscopy methods to reveal new insights in biology and/or medicine and sufficient technical background to design and implement hardware and software solutions that facilitate instrument stability, experimental throughput and Center accessibility as well as teach and disseminate knowledge to non-experts. The mission of the SMC at St. Jude is to push the frontiers of single-molecule methods development across multiple modalities, while maintaining a focus on making these technologies accessible to a broad user base of biology-focused scientists within the department of Structural Biology, and across the Institution and its affiliates. The SMC is integrated into a community of Institutionally-funded laboratories and innovation centers at St. Jude, each with their own dedicated staff, including the Cryo-EM Center, the Protein Technology Center, the Cell and Tissue Imaging Center, The Center for Bioimage Informatics, The Center for Applied Bioinformatics, the Center of Excellence for Data-driven Discovery and the Center for Advanced Genome Engineering (CAGE). The SMC has been founded on advanced confocal and TIRFM single-molecule fluorescence as well as live-cell imaging microscopes with a mission to utilize these technologies for high-impact projects that advance the understanding and treatment of catastrophic childhood diseases, including infectious diseases and cancers. Integrated acquisition and image analysis software is in place. The SMC currently consists of three custom built, sCMOS-based wide-field TIRF microscopes equipped with stopped-flow instrumentation, a modified and elaborated MicroTime200 confocal time-correlated single-photon counting (TCSPC) instrument with 7 laser lines and 4 SPADs together with an ensemble FluoTime300 instrument from PicoQuant, and hybrid live-cell imaging instrumentation. Space and financial support for other types of single-molecule technologies (including optical traps and atomic force microscopes) are available. The SMC is integrated with an on-site machine shop for creating custom-engineered microscope hardware, high-performance computing resources for software development and data management, as well as technology infrastructures for surface chemistry and microfluidics. Technologies within the SMC have enabled discoveries in a wide variety of targetable biological systems, including the cystic fibrosis transmembrane receptor (CFTR), β-arrestins, G-protein coupled receptors (GPCRs), transporters, riboswitches, periplasmic amino acid binding proteins, bacterial/human ribosomes, whole viral particles, and more. In collaborations with the Blanchard laboratory at St. Jude, the SMC also has access to the latest generation of “self-healing” fluorophore technologies and non-natural amino acid incorporation technologies that are both fundamental to advancing the single-molecule imaging field. The successful candidate will continue this history of success by guiding collaborators to achieve single molecule resolution to a wide variety of clinically important biological systems.

The university of Sussex is offering a a 3.5 year Engineering PhD scholarship based in the High Precision and Optical Metrology research lab led by Dr Rodrigo Aviles-Espinosa.

What you get You will receive a tax-free stipend at a standard rate of £19,237 per year and your fees will be waived (at the UK, EU, or International rate). In addition, to a one-off Research and Training Support Grant of £2,000.

PhD project Description: Within the last two decades, multiple efforts have been focused on introducing robotic surgeries in routine procedures including ophthalmology, urology, gynaecology, cardiology, etc. Current commercial robotic systems allow the surgeon to manipulate laparoscopic tools with millimetric precision. However, specialized procedures such as reconstructive microsurgeries, surgical anastomosis, vitreoretinal eye surgery, neurosurgery etc have not yet benefited from robotic surgery given the requirement for sub-millimetre precision considering both the robotic system and surgical tools. This project will bring the fields of ultra short-pulsed laser surgical tools development with high precision robotics to develop the future optical surgical devices delivering submillimetre accuracy for those structures present in specialized procedures (i.e. blood vessels, nerves, cells).

The researcher will design, develop, and test novel laser based surgical devices which will be fully integrated in our existing high precision robotic devices to address the above-mentioned challenges. This will be carried out by:

*Designing optical devices for optimum laser beam delivery considering the surgical task requirements. *Building optical probes capable of providing visualization and manipulation suited for a given micro-surgical task. *Assessing the performance of the developed tool to propose enhancements. *Integrating the optical tool with our high precision robot. *Analysing complex sets of big-data images to extract relevant information using automated strategies.

https://www.sussex.ac.uk/research/centres/robotics-and-mechatronic-systems/research/rodrigo

Eligibility This scholarship is available to UK, EU and overseas applicants. Eligible candidates will have an upper second-class (2:1) undergraduate honours degree (or equivalent qualification) in Physics or Physics Engineering. In addition, to having obtained an an internationally recognised master’s degree (or evidence of its completion in the nearest future) in Physics, Bioengineering, Electrical Engineering or a related field.

Required areas of knowledge in: *Optics and implementation of optical setups and Zemax simulation *Control software and programming skills (LabView, NI-RIO, NI-DAQ, etc.) *Image processing (image J, Matlab, etc) *CAD design.

It will be evaluated favourably if the candidate has experience in spectroscopy and microscopy techniques, and in handling/developing tissue phantoms. Likewise, it will be desirable for the candidate to demonstrate good communication skills and command of the English language. The candidate should hold appropriate expertise from their studies or MSc project to contribute to the research field described above.

The University of Sussex believes that the diversity of its staff and student community is fundamental to creative thinking, pedagogic innovation, intellectual challenge, and the interdisciplinary approach to research and learning. We celebrate and promote diversity, equality and inclusion amongst our staff and students. As such, we welcome applicants from all backgrounds.

Number of scholarships available 1

Deadline 31 May 2024 0:00 How to apply Apply online for a full time PhD in Engineering (SEP2024) using our step-by-step guide.

Please clearly state on your application that you are applying for the Laser Technologies in Medical Applications Scholarship under the supervision of Dr Rodrigo Aviles-Espinosa.

Please ensure you application includes each of the following:

1)A research proposal. For guidance on preparing your proposal, please see here. 2)Degree certificates and transcripts. 3)References, including a minimum of 2 from any institution studied at within the last 5 years. 4)If your first language is not English you will need to demonstrate that you meet the University’s English language requirements, see here for details of our accepted documentation. Contact us For general enquiries, please email phd.engineering@sussex.ac.uk.

For project specific queries, please email r.aviles-espinosa@sussex.ac.uk.

Timetable Application deadline: 31st May 2024 00:00 GMT

Interviews: Mid June 2024

Offers issued to successful candidates: By the 1st July

PhD entry: 16th September 2024

The key objective is to determine how the properties of adeno-associated virus (AAV), such as serotype, capsid stoichiometry, and transgene length, impact the quantity and types of impurities generated. The goal is an improved understanding of the properties of AAV during bioprocessing and how its attributes impact the types of impurities generated. This work will use cutting-edge biophysical techniques, including UCL's time-resolved intrinsic-fluorescence lifetime extraction (TRIFLE) technology, and Cytiva's resin technologies as tools to separate and identify impurities which will then be characterised off-line using a range of techniques including: ddPCR, capsid ELISA, cell-based infectivity assays, and biophysical techniques such as differential scanning fluorimetry, dynamic light scattering and electron microscopy, as appropriate. Further work will address AAV process-related impurities and characterise non-AAV gene therapies including lipid nanoparticle and cell-free synthesis processes.

About us

Founded in 1409, Leipzig University is one of Germany’s largest universities and a leader in research and medical training. With around 30,000 students and more than 5000 members of staff across 14 faculties, it is at the heart of the vibrant and outward-looking city of Leipzig. Leipzig University offers an innovative and international working environment as well as an exciting range of career opportunities in research, teaching, knowledge and technology transfer, infrastructure and administration. The Center for Scalable Data Analytics and Artificial Intelligence Dresden / Leipzig (ScaDS.AI) at Leipzig University seeks to fill the above position at the earliest opportunity. ScaDS.AI Dresden/Leipzig is expanding the national competence center for Big Data, ScaDS, which was founded in 2014. With our research, we aim to bridge the gap between the efficient utilization of massive data and advanced AI. We are developing new methods of machine learning, knowledge management, and methods for preserving privacy. In cooperation with a variety of users from the fields of science and industry, we transfer the developed techniques to various application areas. ScaDS.AI is a research center for Data Science, Artificial Intelligence, and Big Data with locations in Leipzig and Dresden. Both sites are affiliated with a local university - the University of Leipzig and the TU Dresden. As one of five new AI centers in Germany, ScaDS.AI originates back to 2014 and is funded funded since 2019 as part of the federal AI strategy, supported by the Federal Ministry of Education and Research and the Free State of Saxony. You will become part of the German National Research Data Management Initiative for Bioimaging Data (NFDI4BioImage, https://nfdi4bioimage.de/). NFDI4BioImage will strive to provide scientists from all natural science and biomedical research fields with workable and trusted solutions to handle the ever-increasing amount of bioimage data from the various (light) microscopy techniques and related biophotonic technologies. Bioimaging data should thus be in accordance with the FAIR principles, i.e., be Findable, Accessible, Interoperable, and Re-Usable. Increasing data accessibility and re-usability will advance bioimage informatics, the scientific discipline devoted to quantitative analysis and software-aided knowledge extraction from bioimage data. Within this nation-wide network we are responsible for setting up a database and search engine for training materials. About the position

• Fixed term of 3 years
• 100 % of a full-time position. In principle, the position is also suitable for part-time employees. Please note this preference in your application.
• Planned remuneration: salary group E13 TV-L

  Duties

• designing a search index consisting of a database, a search engine and website for Research Data Management, Bio-image Analysis and Data Science related training materials
• implementing and maintaining the software solution
• interacting with developers of similar platforms such as bio.tools, biii.eu and microscopydb.io to make sure our resource is interoperable with these platforms.
• interacting with NFDI4BioImage members and participating scientists guiding them in using our platform
• developing strategies for involving the scientific community in curation and maintenance of the resource
• presenting the resource on national and international conferences

  Requirements

• University degree in data science, computer science, software engineering or related fields
• English and German communication skills (B2)
• Experience with web development (e.g. Drupal, sphinx, Javascript, HTML)
• Experience with ontologies and databases (e.g. EDAM, SPARQL, SQL)
• Experience in Python programming
• Experience in open-source software development is a plus

  What we offer

• A modern workplace and attractive working conditions
• Flexible working hours and work-life balance
• Goal-oriented staff development throughout your working life, with opportunities for continuing professional development
• Pension plan
• a discounted local public transport ticket (e.g. the Deutschlandticket as a job ticket) Please upload your application as a single PDF file. Please note that it is not possible to guarantee confidentiality and rule out unauthorised access by third parties when communicating by unencrypted email. We kindly request that you submit copies only, as we are unable to return application documents. Interview expenses will not be reimbursed. Questions about the position can be addressed to: Dr. Robert Haase, robert.haase@uni-leipzig.de . 0341 97 39309   Leipzig University aims to increase the proportion of women in positions of responsibility and therefore expressly invites qualified women to apply. Severely disabled persons – or persons deemed legally equal to them under Book IX of the German Social Code – are encouraged to apply and will be given preference in the case of equal suitability. 

  Kontakt

  Universität Leipzig Robert Haase Humboldtstraße 25 04105 Leipzig

Tel. 0341 97 39309

The Indiana University School of Medicine is seeking a faculty member at any rank for a tenure track position.  The candidate should be a creative, independent and energetic individual to direct the Indiana Center for Biological Microscopy (ICBM), a 25-year-old core facility whose mission is to promote the effective use of advanced forms of optical microscopy by Indiana University researchers. 
 
The Director will lead a team that provides state-of-art microscopy capabilities to more than 70 Indiana University investigators,100 NIH-funded projects and 3 NIH Centers.  As described on the Center website, (http://web.medicine.iupui.edu/icbm/) the ICBM is equipped with two Leica confocal/multiphoton microscopes, an Olympus confocal/multiphoton microscope, an ISS Fast-FLIM/confocal system, a Nikon live-cell spinning disk confocal microscope, two Akoya Phenocycler (CODEX) multiplexing microscope systems and a Keyence BZX800 high-content/high-throughput imaging system.  Quantitative image analysis is supported with four computer workstations devoted to image analysis (Metamorph, FIJI, Autoquant, Imaris, Halo and Amira).
 
A major focus of the Center is to extend services beyond conventional microscopy, to help investigators implement powerful, but challenging imaging methods, including intravital microscopy, 3D tissue cytometry and advanced methods of image and data analysis.  This is a tenure-track position, and while individuals with funded research programs are welcome to apply, promotion and tenure will primarily be evaluated by the success of the director in the missions to (1) ensure that the ICBM supports the microscopy needs of Indiana University investigators, and (2) actively identify and implement powerful new techniques that will extend their research capabilities.  In addition to a strong background in microscopy and image analysis, successful candidates are expected to have excellent interpersonal skills, and a sincere commitment to customer service.
 
The successful candidate will be expected to
1.    Oversee Center operations, supervising two full-time PhD imaging specialists, who manage Center services, equipment maintenance, system repairs and service billing.
2.    Interact and collaborate with an active community of researchers; provide expert advice in experimental design, facilitate development of new techniques and participate in the preparation of manuscripts.
3.    Contribute to proposals for research funding; help researchers identify appropriate imaging approaches, facilitate the collection of crucial preliminary data and provide text and letters of support.
4.    Develop and realize a strategic vision for service development based upon advances in technology and the evolving needs of the Indiana University research community.
5.    Prepare internal and external grants for acquisition of new imaging equipment. 
6.    Promote the development and support the operation of novel microscopy cores for NIH Center grants.
7.    Promote the opportunities of the Center through targeted seminars presented to IU research groups.
8.    Develop informational materials and teach in microscopy workshops.

Description

Our mission at Micrographia Bio is to deliver a new era of drug discovery using spatial proteomics. We are adding an Engineer in Optical Microscopy into our multidisciplinary team. This role will be responsible for the design, assembly, maintenance, and validation of our optical imaging systems. The successful candidate will join a world-class team that is united by an intrinsic motivation to develop first-in class technology and new medicines. We hire people who do brilliant work and 10X the work of the people around them. As an early team member, you’ll have a major influence over the direction of the company. We’re looking for people who do stunning work. In return we’ll give you an opportunity to grow and develop in a place that’s fun, supportive, and unrelenting in its pursuit of world-class “DeepTech” technology, all while sharing in the success of the company. We think this is a great opportunity for an industrial microscope engineer who is looking to apply their engineering expertise in a start-up industry environment, here in London. If consider building microscopes that unravel biology to be your professional craft, this might be the perfect role for you.

Responsibilities

• Design, build, and characterise of advanced light microscopy systems that drive our technology forward (e.g. optical design, hardware control)
• Collaboratively carry out the design, the acquisition and the analysis of large-scale microscopy experiments in the context of drug discovery
• Communicating theoretical, practical, and experimental knowledge to the company spanning several disciplines (software, AI, commercial)
• Working closely with our inter-disciplinary team to establish integrated fluorescence imaging strategies
• Proactively contributing to a positive, enthusiastic, collaborative engineering culture

  Requirements

  Must haves

• PhD in a relevant technical field (e.g. biophotonics, optics), or equivalent practical experience (advanced microscopy development)
• Experience in assembling / customising fluorescent microscopy imaging systems for specific experimental purposes
• Strong practical and theoretical understanding of optical engineering and fluorescence microscopy
• Ability to think critically and adapt in a fast-paced start-up environment
• Excellent technical communication skills

  Should haves

• Experience in super-resolution microscopy, especially single-molecule imaging
• Software development experience - version control, unix, docker, cloud operations
• Knowledge and experience with Python programing language
• Basic knowledge of cell and molecular biology
• Post-doctoral or industry experience
• Experience in microfabrication

  Nice to haves

• Previous start-up experience
• Experience in core light microscopy facility
• Experience in robotics / control theory

  Benefits

  Salary

• Industry Competitive Salary
• Equity options to share in the company success

  Benefits

• The chance to revolutionise the way we find new drugs and increase probability that you will help discovery drugs that matter with your skillset
• Company matched pension, private healthcare cover, and life insurance
• Fully stocked kitchen with breakfast and snacks
• State-of-the-art London zone 2 working location and laboratories
• The opportunity to work with global thought leaders on our Scientific Advisory Board
• Access to Bubble for Work - for care needs of young children, elderly care, pet care. People first - a culture enabling you to do your best work
• Access to the Cycle-To-Work scheme (including access to secure cycle storage, showers, changing rooms on-site)

  Disclaimers

  Micrographia Bio welcomes applications from all candidates irrespective of age, disability, gender, gender identity, sexual orientation, race, religion or belief, or marital or civil partnership status. If you have a career gap on your CV, but feel motivated by the job description to apply with your skills, we highly encourage your application.

Our research group (Cell Death and Epithelial Homeostasis unit in Institut Pasteur) is seeking a engineer for image analysis (permanent position) to help us developing pipelines for 4D movies analysis and adapt/maintain pipelines previously developped by the group to share them with a large audience. More information can be found here : https://research.pasteur.fr/en/job/image-analysis-engineer-permanent-position-cell-death-and-epithelial-homeostasis-group-institut-pasteur/

Our research group (Cell Death and Epithelial Homeostasis) located in Institut Pasteur (Paris, France) in the department of Developmental and Stem Cell Biology is seeking an engineer in image analysis to join our group through a permanent position, ideally to start in September 2024.

Our group is interested by the fine tuning of cell death in epithelial context using mostly Drosophila as a model system (https://research.pasteur.fr/en/team/cell-death-and-epithelial-homeostasis/)..%20) We use a wide range of live imaging technics and develop new tools (including live sensors and optogenetics) to dissect quantitatively the feedback fine tuning cell death. Our group has been developing over the years various pipelines (z reconstruction, segmentation and tracking, automatic recognition of cellular events through deep learning, force inference…) and is looking for a stable support that will help us to:

-      Develop new pipelines for image analysis mostly for 4D movies (PIV, segmentation, tracking, force inference, features extractions…) -      Maintain and update pipelines previously developed by the group -      Survey newly published methods and adapt them for our needs -      Assist us in the quantitative analysis of our datasets -      Help us to maintain and share our pipelines with a large public through the development of user friendly codes and visual interfaces -      Train the members of the unit for good practice in data management, code writing, metada maintenance and data sharing We are looking for a candidate with good coding knowledge (mostly Python, but also Matlab and Java), good theoretical knowledge of fluorescent live imaging, expertise in machine learning applied to image analysis, a real interest for collaborative work, capacity to work on several projects in parallel and with good listening capacity. The applicant will join a dynamic and supportive group that promotes healthy work life balance, with members coming from various backgrounds, and located in a collegial and vibrant department. The application will also get close interactions with the growing community of image analysts on campus.

If you are interested, please, send a single pdf document with CV, cover letter, Github/Gitlab link, and reference contact to romain.levayer@pasteur.fr (with the subject “engineer position”).

The official job advertisement can be found in the following link: https://emploi.pasteur.fr/job/emploi-ingenieur-en-analyse-d-image-et-donnees-quantitatives-h-f_11427.aspx?LCID=2057

Relevant references: Matamoro-Vidal, A., Cumming, T., Davidovic, A., and Levayer, R. Patterned apoptosis modulates local growth and tissue shape in a fast-growing epithelium. Current Biology 34, 1-13, https://doi.org/10.1016/j.cub.2023.12.031 (2024) Villars, A., Letort, G., Valon, L. & Levayer, R. DeXtrusion: automatic recognition of cell extrusion through machine learning in vivo. Development 150 (13) doi: https://doi.org/10.1242/dev.201747 (2023). Villars, A., Matamoro-Vidal, A., Levillayer, F. & Levayer, R. Microtubule disassembly by caspases is an important rate-limiting step of cell extrusion. Nat Commun 13, 3632, doi: https://doi.org/10.1038/s41467-022-31266-8 (2022). Herbert S.*, Valon L.*, Mancini L., Dray N., Caldarelli P., Gros J., Esposito E., Shorte S.L., Bally-Cuif L., Aulner N., Levayer R., Tinevez J.Y., LocalZProjector and DeProj: a toolbox for local 2D projection and accurate morphometrics of large 3D microscopy images., BMC Biol 2021 Jul; 19(1): 136, doi: https://doi.org/10.1186/s12915-021-01037-w (2021). Valon, L., Davidovic A., Levillayer, F., Villars, A., Chouly, M., Cerqueira-Campos, F., & Levayer, R.* Robustness of epithelial sealing is an emerging property of local ERK feedback driven by cell elimination. Developmental Cell 56, 1-12. doi: https://doi.org/10.1016/j.devcel.2021.05.006 (2021).  Moreno, E., Valon, L., Levillayer, F. & Levayer, R. Competition for Space Induces Cell Elimination through Compaction-Driven ERK Downregulation. Current Biology, 29, 23-34 e28, doi: https://doi.org/10.1016/j.cub.2018.11.007 (2019). 

Postdoctoral position developing a fluorescence lifetime microscope for in vivo imaging of neuron activity and signaling at kilohertz rates. We are developing custom optical systems using electro-optic fluorescence lifetime microscopy (EO-FLIM) to enable fast and quantitative imaging of cellular signals in 3D environments. This work will combine FLIM instrument development, lightsheet microscopy, and applications imaging fluorescent biosensors in vivo.

Position Focus: Senior Staff Scientist position available in the FIB-SEM Collaboration Core (F-SCC) (https://medicine.yale.edu/fib-sem/) of Yale School of Medicine. At the forefront of volume Electron Microscopy, the F-SCC utilizes the state of the art enhanced Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) technology for large volume imaging with nanometer isotropic resolution. Under supervision of the Director of F-SCC, the candidate will play a leadership role in managing enhanced FIB-SEM sample preparation and image acquisition lab within F-SCC. Explicitly, the candidate will be responsible for pipeline processes of X-ray guided FIB-SEM sample preparation, enhanced FIB-SEM image acquisition, and image registration and alignment. The candidate is expected to be multi-tasking, perform independent experiments using the state-of-the-art laboratory techniques including electron, X-ray, and light microscopes. The candidate will work closely with supervisor, group members, and collaborators. While the job duty will evolve according to the needs of the organization, specific responsibilities include: 1.    Perform FIB-SEM sample preparation: mount and trim heavy metal-stained and resin-embedded samples to target specific region of interest using an ultramicrotome guided by X-ray micro-CT. 2.    Perform FIB-SEM image acquisition using customized enhanced FIB-SEM microscopes. Optimize imaging conditions for the best results. Maintain equipment and troubleshoot hardware and software related issues. Perform system installations and upgrades when needed. 3.    Perform 3D image registration and alignment. 4.    Perform IT related system administration duties to maintain image analysis and data storage platform.   Dept/Section URL Click here for more information   Essential Duties 1.    Carries out the wide range of specialized scientific methods and procedures used in the laboratory. 2.    Responsible for resource planning, allocation, and planning renovations. Determines, develops, and implements the operative framework of the laboratory as it relates to long-term goals, needs, and direction. 3.    Keeps abreast of newly-evolving technologies, instrumentation, and methodologies and purchases new instrumentation. 4.    Develops, analyzes, interprets, and summarizes scientific results. 5.    Establishes policies and procedures governing operation of the laboratory. 6.    Independently investigates and solves biotechnological problems as they arise through research and extensive literary searches. Determines subsequent experimental approaches. 7.    Independently decides when additional analyses are needed, arranges for the needed analyses and interprets the resulting design and implements customized protocols. 8.    Authorizes and organizes the use of laboratory and equipment to ensure timely completion of research projects. 9.    May perform other duties as assigned.   Required Education and Experience Master’s Degree in experimental physics, physical chemistry, engineering, or other related discipline and five years of experience or an equivalent combination of experience and education Required Skill/Ability 1: Proficiency in complex instrumentation operation, maintenance, and troubleshooting. Required Skill/Ability 2: Proven dexterity in manipulating small objects and demonstrated ability in three-dimensional thinking. Required Skill/Ability 3: Proficiency in software coding. Familiar with Windows and Linux operating systems. Ability to maintain computers and troubleshoot hardware and software related issues. Ability to learn and carry out IT related system administration duties. Required Skill/Ability 4 Proven ability to search and interpret scientific papers, protocols, standard operating procedures, safety standards, maintenance instructions and procedure manuals for laboratory equipment. Required Skill/Ability 5: Demonstrated capability to manage laboratory facilities and workspaces. Proven ability to plan project needs to optimize resources necessary for successful project completion.   Preferred Education, Experience and Skills: Ph.D. degree in experimental physics, physical chemistry, engineering, or other related discipline, plus six years of relevant experience. Special considerations will be given to those who have expertise in LabVIEW, Python, and MATLAB. Experience in database management (MySQL) and virtual machine environments (VMware ESXi) is a plus.

Internship Microscopy

• Full Time (40 h/w)
• Klosterneuburg near Vienna, Austria
• € 1,906 to € 2,044* gross/month depending on level of education - 3 to 6 months (start in April)

  Your Responsibilities

  The Imaging and Optics Facility of ISTA provides state-of-the-art fluorescence microscopy services, flow cytometry services, as well as custom image analysis and optical development services. As an intern in our team, you will support our scientists in carrying out their research work:

• Contribute to general core facility and infrastructure tasks, including routine facility checks, inventory checks and control of facility (loan) equipment
• Support in basic operation of microscopy equipment
• Assist in standard quality monitoring and performance checks
• Optional: “project involvement” in optimisation of calibration standards and optical maintenance protocols AND/OR involvement in development of advanced imaging controls toolbox (depending on applicant qualification)

  Your Profile

• Completed BSc or enrolled in MSc in Physics, Life Science or Neuroscience (or equivalent)
• Basic knowledge in microscopy techniques (e.g. Brightfield, Fluorescence, Confocal)
• Basic knowledge of programming languages (e.g. FIJI macro, Matlab, Python) of advantage
• High level of interest in the Imaging and Optics Facility services
• Knowledge of programming/scripting languages (e.g. C, Python) required for “project involvement” in development of advanced imaging controls toolbox
• Strong affinity with Optics, Electronics and Programming of advantage for “project involvement”
• Service-oriented and conscientious approach to work
• Eager to learn, proactive personality and team player
• Ability to independently take over small projects
• Good command of English (Institute language)

  ISTA is growing. Grow with us!

  Institute of Science and Technology Austria (ISTA) is a constantly growing international institute for conducting frontier research in mathematics, computer science, life sciences & physical sciences. We strive to recruit passionate professionals from across the world over all fields who strive to support our goal of excellent research. Located within a beautiful campus on the outskirts of Vienna, we offer multiple opportunities for personal growth in a stable working environment. Get an insight! *This position comes with possible overpayment depending on education, qualification and work experience. For more information on how ISTA processes your personal data, please refer to www.ista.ac.at/data-protection.

We are recruiting a new director for the Biological Imaging Development CoLab (BIDC) at the University of California in San Francisco.

CoLabs (http://colabs.ucsf.edu/) is a new model for supporting collaborative research at UCSF. The BIDC is one of five CoLabs teams with specialized research and technical expertise in analyzing biological samples using various approaches. These teams work together closely and collaborate with a large group of investigators in many UCSF departments. BIDC’s missions include microscopy education, training, consulting, and technical support and microscopy research and development.

The BIDC Director role is multifaceted and requires independence, initiative, organization, and flexibility. The incumbent must have experience in biological-related microscopy research, strong interpersonal skills to work closely with investigators, students, postdoctoral fellows, visiting scholars, and staff members, and strong time management skills. The incumbent also must have strong mentoring and leadership skills.

In general, the BIDC Director’s activities will include, but are not limited to: maintenance and management of the Biological Imaging Development CoLab’s (BIDC) microscopes and support equipment; training and assisting new users on microscopes, techniques, and data analysis; assisting in experimental design and analysis pipelines; direct supervision of two or more employees; oversight of CoLab’s budget including salaries and materials; coordinating team members for new initiatives and projects; and the mentoring and career development of co-workers. The BIDC Director is also responsible for keeping the facility current with trends and needs of the microscopy community. This is accomplished either through the raising funds and purchasing commercial equipment, designing and developing technology, or adding new support resources for the research community. The incumbent will report directly to the Director of the CoLabs and the BIDC Steering Committee.

Required qualifications:

A PhD in natural sciences; biology, chemistry, physics, or related discipline. At least 5 years of experience in microscopy-based instrument development. At least 5 years of experience in high-dimensional microscopy data analysis. At least 5 years of experience in biology specific microscopy techniques and analysis. Proficiency in microscopy data analysis using available software such as Imaris, Elements, LAS, FIJI, ImageJ, and Ilastik. Demonstrated skills and experience in teaching and mentoring graduate students and professional trainees. Strong interpersonal skills and the ability to work with individuals from diverse backgrounds. Strong time management skills and demonstrated attention to details. Preferred qualifications:

Excellent written and verbal communication including writing of research manuscripts, and scientific oral presentations (in-person and virtually). Experience in preparing grant proposals, especially shared equipment grant proposals. Experience in management of a shared research resource or core facility. Proficiency in custom microscopy data analysis using Matlab and Python. Hands-on experience in confocal, widefield, light-sheet, and super-resolution microscopy techniques. Experience in computer assisted drawing (CAD) for instrument design and development. Experience in electrical circuit design with soldering skills.